Simultaneous Treatment of Long-chain Monounsaturated Fatty Acid and n-3 Polyunsaturated Fatty Acid Decreases Lipid and Cholesterol Levels in HepG2 Cell.

Kazuaki Yoshinaga, Yota Mizuno, Samanthika Senarath, Aya Yoshinaga-Kiriake, Toshiharu Nagai, Fumiaki Beppu, Seiya Tanaka, Naohiro Gotoh

Journal of oleo science 2021 May 01

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The n-3 type polyunsaturated fatty acids (n-3PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), from fish oil exhibit health benefits such as triacylglycerol- and cholesterol-lowering effects. Some pelagic fishes contain long-chain monounsaturated fatty acids (LC-MUFAs) such as eicosenoic acid (C20:1), which exert health-promoting effects. However, no study has evaluated beneficial effects of n-3PUFA and LC-MUFA combination. Here, we investigated effects of simultaneous treatment with n-3PUFA (EPA and DHA) and LC-MUFA (cis-5-C20:1 and cis-7-C20:1) and found that n-3PUFA and LC-MUFA combination significantly decreased lipid accumulation and reduced total cholesterol in HepG2 cells. Cholesterol level was significantly lower in DHA + cis-7-C20:1 group than in DHA + EPA group. These results suggest the importance of LC-MUFA as a functional molecule in fish oil.

Citation

Kazuaki Yoshinaga, Yota Mizuno, Samanthika Senarath, Aya Yoshinaga-Kiriake, Toshiharu Nagai, Fumiaki Beppu, Seiya Tanaka, Naohiro Gotoh. Simultaneous Treatment of Long-chain Monounsaturated Fatty Acid and n-3 Polyunsaturated Fatty Acid Decreases Lipid and Cholesterol Levels in HepG2 Cell. Journal of oleo science. 2021 May 01;70(5):731-736