Rodent incisor and molar dental follicles show distinct characteristics in tooth eruption.

Rodent incisors and molars show different eruption patterns. Dental follicles and their interaction with dental epithelia play key roles in tooth eruption. However, little is known about the differences between incisor dental follicle (IF) and molar dental follicle (MF) during tooth eruption of rodents. This study aimed to investigate the differences between IF and MF during tooth eruption under induction with cervical-loop cells (CLC) and Hertwig's epithelial root sheath (HERS) cells of rats. CLC, HERS, IF, MF cells were isolated from 10 postnatal day 7 rats and identified by immunofluorescence staining. CLC or HERS cells-derived conditioned medium (CM) was obtained to induce IF and MF cells. Cell proliferation, mineralization, gene and protein expression related to tooth eruption were detected, and histological analysis was also performed. The osteogenic differentiation and mineralization abilities of IF cells were stronger than those of MF cells. Both CLC and HERS cells-derived CM enhanced these abilities of IF cells, whereas they showed the opposite effect on MF cells. At 7, 10, and 15 d after birth, IF cells expressed more OPG and less RANKL than MF cells. IF and MF cells present distinct characteristics in tooth eruption, CLC and HERS cells have significant inductive effects on them. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Mengting He, Peiqi Wang, Bin Li, Yipeng Wang, Xinghai Wang, Ding Bai, Yongwen Guo. Rodent incisor and molar dental follicles show distinct characteristics in tooth eruption. Archives of oral biology. 2021 Jun;126:105117

Mesh Tags

PMID: 33845260

search → result