Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay.

Per H Nilsson, Kristin Pettersen, Martin Oppermann, Espen W Skjeflo, Hilde Fure, Dorte Christiansen, Tom Eirik Mollnes

Methods in molecular biology (Clifton, N.J.) 2021

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Enzyme-linked immunosorbent assay (ELISA) enables fast and simple quantification of analytes in the pico- to nanogram range in complex samples. Here, we describe an ELISA for the detection of porcine C3a as a marker for complement activation. Antibody specificity is critical for a robust assay. This assay is based on a pair of antibodies specific for the porcine C3a molecule and thus does not react with native C3.

Citation

Per H Nilsson, Kristin Pettersen, Martin Oppermann, Espen W Skjeflo, Hilde Fure, Dorte Christiansen, Tom Eirik Mollnes. Quantification of Porcine Complement Activation Fragment C3a by a Neoepitope-Based Enzyme-Linked Immunosorbent Assay. Methods in molecular biology (Clifton, N.J.). 2021;2227:51-59