In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope.

Xiaofei Qin, Chong Chen, Linbo Wang, Xiaohu Chen, Yong Liang, Xin Jin, Weijun Pan, Zhiying Liu, Hui Li, Guang Yang

Biochemical and biophysical research communications 2021 Jun 11

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Precise quantification of vascular developments in Zebrafish requires continuous in-vivo 3D imaging. Here we employed a bi-directional light-sheet illumination microscope to characterize the development process of Zebrafish's intersegmental vessels. A Virtual Reality-based method was used to measure the lengths of intersegmental vessels (ISVs). The quantified growth rates of typical ISVs can be plotted, and unusual growth of some specific vessels was also observed. Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Xiaofei Qin, Chong Chen, Linbo Wang, Xiaohu Chen, Yong Liang, Xin Jin, Weijun Pan, Zhiying Liu, Hui Li, Guang Yang. In-vivo 3D imaging of Zebrafish's intersegmental vessel development by a bi-directional light-sheet illumination microscope. Biochemical and biophysical research communications. 2021 Jun 11;557:8-13