Protocol for analysis of integrin-mediated cell adhesion of lateral plate mesoderm cells isolated from zebrafish embryos.

Seung-Sik Rho, Eri Oguri-Nakamura, Koji Ando, Kiyotake Yamamoto, Yuki Takagi, Shigetomo Fukuhara

STAR protocols 2021 Jun 18

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Lateral plate mesoderm (LPM) cells differentiate into various cell types including endothelial and hematopoietic cells. In zebrafish embryos, LPM cells migrate toward the midline along the ventral surfaces of somites during which their cell fate specification depends upon efficient integrin-mediated cell adhesion and migration. Herein, we present a protocol for analysis of integrin-mediated cell adhesion of LPM cells isolated from zebrafish embryos. This allows the study of the molecular mechanisms underlying integrin activation required for LPM cell fate specification. For complete details on the use and execution of this protocol, please refer to Rho et al. (2019). © 2021 The Author(s).

Citation

Seung-Sik Rho, Eri Oguri-Nakamura, Koji Ando, Kiyotake Yamamoto, Yuki Takagi, Shigetomo Fukuhara. Protocol for analysis of integrin-mediated cell adhesion of lateral plate mesoderm cells isolated from zebrafish embryos. STAR protocols. 2021 Jun 18;2(2):100428