Cryo-EM structure of human ABCB8 transporter in nucleotide binding state.

Shunjin Li, Yue Ren, Xuhang Lu, Yuequan Shen, Xue Yang

Biochemical and biophysical research communications 2021 Jun 11

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Human ATP-binding cassette transporter 8 of subfamily B (hABCB8) is an ABC transporter that located in the inner membrane of mitochondria. The ABCB8 is involved in the maturation of Fe-S and protects the heart from oxidative stress. Here, we present the cryo-EM structure of human ABCB8 binding with AMPPNP in inward-facing conformation with resolution of 4.1 Å. hABCB8 shows an open-inward conformation when ATP is bound. Unexpectedly, cholesterol molecules were identified in the transmembrane domain of hABCB8. Our results provide structural basis for the transport mechanism of the ABC transporter in mitochondria. Copyright © 2021 Elsevier Inc. All rights reserved.

Citation

Shunjin Li, Yue Ren, Xuhang Lu, Yuequan Shen, Xue Yang. Cryo-EM structure of human ABCB8 transporter in nucleotide binding state. Biochemical and biophysical research communications. 2021 Jun 11;557:187-191