Correlation Engine 2.0
Clear Search sequence regions


  • acids (9)
  • cellular (1)
  • inulin (5)
  • lactobacillus (3)
  • rnase (1)
  • Sizes of these terms reflect their relevance to your search.

    Ribonucleic acids (RNA) are involved in many cellular functions. In general, RNA is made up by only four different ribonucleotides. The modifications of RNA (epitranscriptome) can greatly enhance the structural diversity of RNA, which in turn support some of the RNA functions. To determine whether the epitranscriptome of a specific probiotic is associated with its adaptation to the source of energy, Lactobacillus agilis (YZ050) was selected as a model and its epitranscriptome was profiled and compared by using mass spectrometry. The L. agilis epitranscriptome (minus rRNA modifications) consists of 17 different RNA modifications. By capturing the L. agilis cells during exponential growth, reproducible profiling was achieved. In a comparative study, the standard source of energy (glucose) in the medium was substituted by a prebiotic inulin, and a downward trend in the L. agilis epitranscriptome was detected. This marks the first report on a system-wide variation of a bacterial epitranscriptome that resulted from adapting to an alternative energy source. No correlation was found between the down-regulated RNA modifications and the expression level of corresponding writer genes. Whereas, the expression level of a specific exonuclease gene, RNase J1, was detected to be higher in cells grown on inulin.

    Citation

    Hongzhou Wang, Jennifer H Simpson, Madison E Kotra, Yuanting Zhu, Saumya Wickramasinghe, David A Mills, Norman H L Chiu. Epitranscriptomic profile of Lactobacillus agilis and its adaptation to growth on inulin. BMC research notes. 2021 Apr 21;14(1):154

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 33883017

    View Full Text