Characteristics of Gd(III) spin labels for the study of protein conformations.

Gd(III) complexes are currently established as spin labels for structural studies of biomolecules using pulse dipolar electron paramagnetic resonance (PD-EPR) techniques. This has been achieved by the availability of medium- and high-field spectrometers, understanding the spin physics underlying the spectroscopic properties of high spin Gd(III) (S=7/2) pairs and their dipolar interaction, the design of well-defined model compounds and optimization of measurement techniques. In addition, a variety of Gd(III) chelates and labeling schemes have allowed a broad scope of applications. In this review, we provide a brief background of the spectroscopic properties of Gd(III) pertinent for effective PD-EPR measurements and focus on the various labels available to date. We report on their use in PD-EPR applications and highlight their pros and cons for particular applications. We also devote a section to recent in-cell structural studies of proteins using Gd(III), which is an exciting new direction for Gd(III) spin labeling. Copyright © 2021 Elsevier Inc. All rights reserved.

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Angeliki Giannoulis, Yasmin Ben-Ishay, Daniella Goldfarb. Characteristics of Gd(III) spin labels for the study of protein conformations. Methods in enzymology. 2021;651:235-290

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PMID: 33888206

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