A Facile Aptasensor for Instantaneous Determination of Cadmium Ions Based on Fluorescence Amplification Effect of MOPS on FAM-Labeled Aptamer.

Yang Liu, Dongwei Zhang, Jina Ding, Kashif Hayat, Xijia Yang, Xuejia Zhan, Dan Zhang, Yitong Lu, Pei Zhou

Biosensors 2021 Apr 23

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Analytical performance and efficiency are two pivotal issues for developing an on-site and real-time aptasensor for cadmium (Cd2+) determination. However, suffering from redundant preparations, fabrications, and incubation, most of them fail to well satisfy the requirements. In this work, we found that fluorescence intensity of 6-carboxyfluorescein(FAM)-labeled aptamer (FAM-aptamer) could be remarkably amplified by 3-(N-morpholino)propane sulfonic acid (MOPS), then fell proportionally as Cd2+ concentration introduced. Importantly, the fluorescence variation occurred immediately after addition of Cd2+, and would keep stable for at least 60 min. Based on the discovery, a facile and ultra-efficient aptasensor for Cd2+ determination was successfully developed. The sensing mechanism was confirmed by fluorescence pattern, circular dichroism (CD) and intermolecular interaction related to pKa. Under the optimal conditions, Cd2+ could be determined rapidly from 5 to 4000 ng mL-1. The detection limit (1.92 ng mL-1) was also lower than the concentration limit for drinking water set by WHO and EPA (3 and 5 ng mL-1, respectively). More than a widely used buffer, MOPS was firstly revealed to have fluorescence amplification effect on FAM-aptamer upon a given context. Despite being sensitive to pH, this simple, high-performance and ultra-efficient aptasensor would be practical for on-site and real-time monitoring of Cd2+.

Citation

Yang Liu, Dongwei Zhang, Jina Ding, Kashif Hayat, Xijia Yang, Xuejia Zhan, Dan Zhang, Yitong Lu, Pei Zhou. A Facile Aptasensor for Instantaneous Determination of Cadmium Ions Based on Fluorescence Amplification Effect of MOPS on FAM-Labeled Aptamer. Biosensors. 2021 Apr 23;11(5)