The Roots of Rye (Secale cereale L.) Are Capable of Synthesizing Benzoxazinoids.

Monika Rakoczy-Trojanowska, Bartosz M Szabała, Elżbieta Różańska, Mariusz Kowalczyk, Wojciech Burza, Beata Bakera, Magdalena Święcicka

International journal of molecular sciences 2021 Apr 28

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According to current opinion, the first step of benzoxazinoids (BXs) synthesis, that is, the conversion of indole-3-glycerol phosphate to indole, occurs exclusively in the photosynthesising parts of plants. However, the results of our previous work and some other studies suggest that this process may also occur in the roots. In this study, we provide evidence that the first step of BXs synthesis does indeed occur in the roots of rye seedlings. We detected ScBx1 transcripts, BX1 enzyme, and six BXs (2-hydroxy-1,4-benzoxazin-3-one, 2,4-dihydroxy-1,4-benzoxazin-3-one, (2R)-2-O-β-d-glucopyranosyl-4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one glucoside, 2,4-dihydroxy- 7-methoxy-1,4-benzoxazin-3-one, 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside, and 6-methoxy-2-benzoxazolinone) in the roots developed from seeds deprived of the coleoptile at 2 days after sowing (i.e., roots without contact with aerial parts). In roots regenerated in vitro, both ScBx1 transcripts and BX1 enzyme were detected at a low but still measurable levels. Thus, BXs are able to be synthesised in both the roots and above-ground parts of rye plants.

Citation

Monika Rakoczy-Trojanowska, Bartosz M Szabała, Elżbieta Różańska, Mariusz Kowalczyk, Wojciech Burza, Beata Bakera, Magdalena Święcicka. The Roots of Rye (Secale cereale L.) Are Capable of Synthesizing Benzoxazinoids. International journal of molecular sciences. 2021 Apr 28;22(9)