CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells.

Nature biotechnology 2022 Feb

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Prime editors have been delivered using DNA or RNA vectors. Here we demonstrate prime editing with purified ribonucleoprotein complexes. We introduced somatic mutations in zebrafish embryos with frequencies as high as 30% and demonstrate germline transmission. We also observed unintended insertions, deletions and prime editing guide RNA (pegRNA) scaffold incorporations. In HEK293T and primary human T cells, prime editing with purified ribonucleoprotein complexes introduced desired edits with frequencies of up to 21 and 7.5%, respectively. © 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.

Citation

Karl Petri, Weiting Zhang, Junyan Ma, Andrea Schmidts, Hyunho Lee, Joy E Horng, Daniel Y Kim, Ibrahim C Kurt, Kendell Clement, Jonathan Y Hsu, Luca Pinello, Marcela V Maus, J Keith Joung, Jing-Ruey Joanna Yeh. CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells. Nature biotechnology. 2022 Feb;40(2):189-193