Identification of Follicular T-Cell Subsets in Murine and Human Tissues.

Annette Lahmann, Laura Bauer, Andreas Hutloff

Methods in molecular biology (Clifton, N.J.) 2021

filter terms:

Follicular helper T (Tfh) cells play a key role in B cell activation and differentiation. Within recent years, distinct subsets of follicular T cells, including regulatory and cytotoxic T cells, have been identified. Apart from classical Tfh cells in secondary lymphoid organs, Tfh-like cells are found in chronically inflamed nonlymphoid tissues. Here, we provide protocols to identify different follicular T cell subsets in murine and human tissues by flow cytometry. This chapter also contains an immunization protocol for the induction of large numbers of Tfh cells in mice.

Citation

Annette Lahmann, Laura Bauer, Andreas Hutloff. Identification of Follicular T-Cell Subsets in Murine and Human Tissues. Methods in molecular biology (Clifton, N.J.). 2021;2285:77-90