Gamma radiation effects on diamond field-effect biosensors with fibroblasts and extracellular matrix.

Marie Krátká, Jan Čermák, Jana Vachelová, Marie Davídková, Nataliya Romanyuk, Alexander Kromka, Bohuslav Rezek

Colloids and surfaces. B, Biointerfaces 2021 Aug

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Due to high biocompatibility, miniaturization, optical transparency and low production cost together with high radiation hardness the diamond-based sensors are considered promising for radiation medicine and biomedicine in general. Here we present detection of fibroblast cell culture properties by nanocrystalline diamond solution-gated field-effect transistors (SG-FET), including effects of gamma irradiation. We show that blank nanocrystalline diamond field-effect biosensors are stable at least up to 300 Gy of γ irradiation. On the other hand, gate current of the diamond SG-FET biosensors with fibroblastic cells increases exponentially over an order of magnitude with increasing radiation dose. Extracellular matrix (ECM) formation is also detected and analyzed by correlation of electronic sensor data with optical, atomic force, fluorescence, and scanning electron microscopies. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Marie Krátká, Jan Čermák, Jana Vachelová, Marie Davídková, Nataliya Romanyuk, Alexander Kromka, Bohuslav Rezek. Gamma radiation effects on diamond field-effect biosensors with fibroblasts and extracellular matrix. Colloids and surfaces. B, Biointerfaces. 2021 Aug;204:111689