Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing.

Xiya Zhang, Tao Li, Jianping Ou, Junjiu Huang, Puping Liang

Protein & cell 2022 May

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Recent advances in genome editing, especially CRISPR-Cas nucleases, have revolutionized both laboratory research and clinical therapeutics. CRISPR-Cas nucleases, together with the DNA damage repair pathway in cells, enable both genetic diversification by classical non-homologous end joining (c-NHEJ) and precise genome modification by homology-based repair (HBR). Genome editing in zygotes is a convenient way to edit the germline, paving the way for animal disease model generation, as well as human embryo genome editing therapy for some life-threatening and incurable diseases. HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template. Here, we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor. Moreover, we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos. Finally, we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system. © 2021. The Author(s).

Citation

Xiya Zhang, Tao Li, Jianping Ou, Junjiu Huang, Puping Liang. Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing. Protein & cell. 2022 May;13(5):316-335