Validated B vitamin quantification from lentils by selected reaction monitoring mass spectrometry.

A validated method for B vitamin separation and quantification from lentil seeds using ultra high performance liquid chromatography-selected reaction monitoring mass spectrometry (UHPLC-SRM MS) was reported. The use of three enzymes (acid phosphatase, β-glucosidase, and rat serum) with a 4 h incubation was sufficient to convert bound B vitamins into their free forms. Twenty B vitamers were selected and a 5-min UHPLC-SRM MS method was optimized for rapid analysis. This method was applied to quantify B vitamin concentration during lentil seed germination over a 5-day period. Total B vitamins increased up to 1.5-fold on day 5 (from 39.2 µg/g to 60.6 µg/g of dry weight) comparing with dry seeds. Vitamin B5 (pantothenic acid) was the most abundant B vitamin in both dry seeds (34.2%) and in germinated seeds (17.7%-24.5% of total B vitamins); B8 (biotin) and B12 (cyanocobalamin) were not detected in lentil samples. Copyright © 2021 Elsevier Ltd. All rights reserved.

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Haixia Zhang, Devini De Silva, Dilanganie Dissanayaka, Thomas D Warkentin, Albert Vandenberg. Validated B vitamin quantification from lentils by selected reaction monitoring mass spectrometry. Food chemistry. 2021 Oct 15;359:129810

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PMID: 33957327

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