Mingyue Ai, Chuanjia Guo, Liwei Wang, Ming Hu, Kaixu Xu, Chen Li, Zhimin Zhou
Colloids and surfaces. B, Biointerfaces 2021 AugIt is important to achieve precise surface charge manipulation of non-spherical drug microcrystals using facile and time-efficient methods for local drug delivery. In this study, silk-coated dexamethasone (DEX) non-spherical microcrystals were synthesized by precipitation technique followed by alternate deposition of poly(allylamine hydrochloride) (PAH) (or PAH-coated Fe3O4) and silk fibroin (SF) via layer-by-layer assembly. EDC and glutaraldehyde were employed to manipulate positive or negative charge of particles by simple chemical cross-linking reactions, respectively. In vivo assessment was carried out by intratympanic (IT) injection of DEX non-spherical microcrystals in guinea pigs. In vivo pharmacokinetic results demonstrate that negatively charged DEX microcrystals appeared to improve outcomes of inner ear delivery in comparison to positively-charged counterparts. This is partly because of the adhesive features of the SF. The present study may provide new ideas to construct surface charge-tunable drug delivery vehicles that are capable of crossing biological barriers, especially for inner ear delivery due to the simple and practical strategy. Copyright © 2021 Elsevier B.V. All rights reserved.
Mingyue Ai, Chuanjia Guo, Liwei Wang, Ming Hu, Kaixu Xu, Chen Li, Zhimin Zhou. Modulating surface charge of dexamethasone non-spherical microcrystals for improved inner ear delivery. Colloids and surfaces. B, Biointerfaces. 2021 Aug;204:111806
PMID: 33957492
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