Correlation Engine 2.0
Clear Search sequence regions

  • ADCY1 (1)
  • ARRB1 (1)
  • ATP1A2 (1)
  • BAIAP2 (1)
  • CA12 (1)
  • cancer (1)
  • DECR1 (1)
  • esophagus squamous cell carcinoma (3)
  • FABP4 (1)
  • gene (3)
  • GNA15 (2)
  • GNAI1 (1)
  • GNAZ (1)
  • humans (1)
  • IL1RAPL2 (2)
  • LAD1 (1)
  • LDOC1 (1)
  • mrnas (11)
  • PPARD (1)
  • PRKAR2B (1)
  • RAP2B (1)
  • rna (4)
  • SH3GL1 (1)
  • squamous cell carcinoma (1)
  • TSPAN10 (1)
  • ZNF853 (1)
  • Sizes of these terms reflect their relevance to your search.

    The goal of our study was to screen tumor grade-related lncRNAs and mRNAs to reveal the underlying molecular mechanism of esophagus squamous cell carcinoma (ESCC). The lncRNA and mRNA sequencing data were obtained from The Cancer Genome Atlas (TCGA). Tumor grade correlation analysis of lncRNAs and mRNAs was executed, followed by the functional enrichment analysis of all tumor grade-related mRNAs. The differentially expression mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs) were obtained. PPI network and DEmRNA-DElncRNA interaction analysis were constructed. The functional annotation of the DEmRNAs co-expressed with DElncRNAs was performed. The expression levels of the candidate genes were validated using qRT-PCR. A total of 1864 tumor grade-related mRNAs (846 positively related and 1018 negatively related) and 552 tumor grade-related lncRNAs (331 positively related and 221 negatively related) were obtained. The top 10 significantly grade-related mRNAs and lncRNAs included CA12, FABP4, DECR1, BAIAP2, IL1RAPL2, PPARD, LAD1, TSPAN10, LDOC1, ZNF853, RP11-25G10.2, RP11-557H15.3, RP11-521D12.5, CHKB-AS1, RP11-219B4.3, CH17-335B8.4, RP11-99 J16-A.2, CTB-111H14.1, ADNP-AS1, and JHDM1D-AS1. SFN, IL1RAPL2, and RP11-25G10.2 were overlapped from grade 1, grade 2, and grade 3. PPI network showed that top 10 proteins with higher degrees, including GNAI1, RAP2B, GNAZ, SHH, ADCY1, PRKAR2B, SH3GL1, GNA15, and ARRB1. A DElncRNAs-nearby DEmRNAs network was constructed to obtain hub lncRNAs including ADAMTS9-AS2, RP11-210 M15.2, RP11-13 K12.1, ZBED3-AS1, and RP11-25G10.2. Except for RP11-25G10.2, ADAMTS9-AS1, ZBED3-AS1, SFN, ATP1A2, and GNA15 were consistent with our TCGA analysis. Alterations of DEmRNAs and DElncRNAs may provide key insights into the molecular mechanisms of ESCC. © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.


    Xin Gao, Qian Liu, Xue Chen, Shaoping Chen, Jianmei Yang, Qiang Liu, Yufeng Cheng. Screening of tumor grade-related mRNAs and lncRNAs for Esophagus Squamous Cell Carcinoma. Journal of clinical laboratory analysis. 2021 Jun;35(6):e23797

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 33960436

    View Full Text