Mechanisms and kinetics for the degradation of paclobutrazol and biocontrol action of a novel Pseudomonas putida strain T7.

The present study explores biodegradation kinetics and process optimization of plant growth retardant from triazole group paclobutrazol (PBZ; C15H20ClN3O mol. wt. 293.79 g mol-1) in a batch experiment. A gram-negative rod-shaped bacterium T7 was isolated from PBZ applied agricultural field by enrichment technique and characterized as Pseudomonas putida strain T7. Strain was tested for PBZ biodegradation and plant growth-promoting characteristics. Results revealed that strain T7 utilizes PBZ as a carbon and energy source and showing degradation up to 98.30% on the 15th day. First-order degradation kinetics and a linear model were well fitted and showing a maximum t1/2 value on 9th day. Biodegradation optimization by Box Behnken design (BBD) of Response surface methodology (RSM) showed maximum degradation at pH 7.0, 31 °C temperature, and 2 mL inoculum size (8 × 109 CFU mL-1). The bacterium was also able to solubilize Zn, K, and PO4 and produced a copious amount of IAA, HCN, and Ammonia. The biocontrol activity against plant pathogens like Fusarium oxysporum (MTCC-284), Colletotrichum gloeosporioides (MTCC 2190), Pythium aphanidermatum (MTCC - 1024), Tropical race-1 (TR -1), and Tropical race - 4 (TR-4) showed the great antagonistic effect. Hence, this strain can be employed as an effective bio-agent for eco-friendly cleanup strategies and pathogen suppressive agents in paclobutrazol contaminated soil. Copyright © 2021 Elsevier Inc. All rights reserved.

Citation

Govind Kumar, Shatrohan Lal, Pankaj Bhatt, R A Ram, A K Bhattacherjee, Abhay Dikshit, Shailendra Rajan. Mechanisms and kinetics for the degradation of paclobutrazol and biocontrol action of a novel Pseudomonas putida strain T7. Pesticide biochemistry and physiology. 2021 Jun;175:104846

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PMID: 33993964

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