Correlation Engine 2.0
Clear Search sequence regions


  • 53BP1 (1)
  • autism (4)
  • behaviors (1)
  • brain (1)
  • cellular (1)
  • Ddit4 (1)
  • dna repair (3)
  • impairs (2)
  • KMT5B (12)
  • mice (2)
  • phenotypes (1)
  • receptor (1)
  • Redd1 (1)
  • rna (1)
  • subunits proteins (1)
  • target gene (1)
  • ubiquitin (1)
  • ubiquitin- protein (1)
  • Sizes of these terms reflect their relevance to your search.

    Large-scale genetic screening has identified KMT5B (SUV420H1), which encodes a histone H4 K20 di- and tri-methyltransferase highly expressed in prefrontal cortex (PFC), as a top-ranking high-risk gene for autism. However, the biological function of KMT5B in the brain is poorly characterized, and how KMT5B deficiency is linked to autism remains largely unknown. Here we knocked down Kmt5b in PFC and examined behavioral and electrophysiological changes, as well as underlying molecular mechanisms. Mice with Kmt5b deficiency in PFC display social deficits, a core symptom of autism, without the alteration of other behaviors. Kmt5b deficiency also produces deficits in PFC glutamatergic synaptic transmission, which is accompanied by the reduced synaptic expression of glutamate receptor subunits and associated proteins. Kmt5b deficiency-induced reduction of H4K20me2 impairs 53BP1-mediated DNA repair, leading to the elevation of p53 expression and its target gene Ddit4 (Redd1), which is implicated in synaptic impairment. RNA-sequencing data indicate that Kmt5b deficiency results in the upregulation of genes enriched in cellular stress response and ubiquitin-dependent protein degradation. Collectively, this study has revealed the functional role of Kmt5b in the PFC, and suggests that Kmt5b deficiency could cause autistic phenotypes by inducing synaptic dysfunction and transcriptional aberration. © 2021. The Author(s), under exclusive licence to American College of Neuropsychopharmacology.

    Citation

    Zi-Jun Wang, Ben Rein, Ping Zhong, Jamal Williams, Qing Cao, Fengwei Yang, Freddy Zhang, Kaijie Ma, Zhen Yan. Autism risk gene KMT5B deficiency in prefrontal cortex induces synaptic dysfunction and social deficits via alterations of DNA repair and gene transcription. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology. 2021 Aug;46(9):1617-1626

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 34007043

    View Full Text