Mutation and Copy Number Alterations Analysis of KIF23 in Glioma.

In glioma, kinesin family member 23 (KIF23) is up-regulated and plays a vital role in oncogenesis. However, the mechanism underlying KIF23 overexpression in malignant glioma remains to be elucidated. This study aims to find potential causes of KIF23 high expression at genome level. To clarify this issue, we obtained point mutation and copy number alterations (CNAs) of KIF23 in 319 gliomas using whole-exome sequencing. Only two glioma samples with missense mutations in KIF23 coding region were identified, while 7 patients were detected with amplification of KIF23. Additional analysis showed that KIF23 amplification was significantly associated with higher expression of KIF23. Gene ontology analysis indicated that higher copy number of KIF23 was associated TNF-α signaling pathway and mitotic cell circle checkpoint, which probably caused by subsequent upregulated expression of KIF23. Moreover, pan-cancer analysis showed that gaining of copy number was significantly associated with higher expression of KIF23, consolidating our findings in glioma. Thus, it was deduced that elevated KIF23 expression in glioma tended to be caused by DNA copy number amplification, instead of mutation. Copyright © 2021 Zhao, Wang, Bao, Gao, Zhang, Ruan, Lv, Wang and Sun.

Citation

Zheng Zhao, Zheng Wang, Zhao-Shi Bao, Wei-Zhen Gao, Yuan-Da Zhang, Ci-Jie Ruan, Tao Lv, Yong Wang, Li-Hua Sun. Mutation and Copy Number Alterations Analysis of KIF23 in Glioma. Frontiers in genetics. 2021;12:646929

PMID: 34017355

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