The promising pro-healing role of the association of mesoglycan and lactoferrin on skin lesions.

Raffaella Belvedere, Emanuela Pessolano, Nunzia Novizio, Alessandra Tosco, Daniela Eletto, Amalia Porta, Amelia Filippelli, Francesco Petrella, Antonello Petrella

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2021 Aug 01

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Skin wound repair represents an important topic for the therapeutic challenges. Many molecules are commonly used as active principles of topical devices to induce the correct tissue regeneration. Among these molecules, mesoglycan, a mixture of glycosaminoglycans, and the lactoferrin have recently aroused interest. Here, for the first time, we used mesoglycan/lactoferrin to treat the cell populations mainly involved in wound healing. We showed that human keratinocytes, fibroblasts and endothelial cells migrate and invade more rapidly when treated with the association. Moreover, we found that mesoglycan/lactoferrin, are able to trigger the differentiation process of keratinocytes, the switch of the fibroblasts into myofibroblasts, the acquisition of a mesenchymal phenotype for the endothelial cells which, in this way, start to form the capillary-like structures. Additionally, we proved that the well known antimicrobial behavior of lactoferrin encourages the inhibition of S. aureus and P. aeruginosa biofilm formation by the whole association, providing an appealing feature for this formulation. Finally, by the in vivo analysis, we showed that the mesoglycan/lactoferrin favors the closure of skin wounds performed on the mice back. Beside the decrease of the lesion diameters, by a confocal analysis of mice biopsies we found that the use of the association strongly promote cell activation underlying the correct tissue regeneration. These results encourage to further investigation aiming the development of a new topical patch that includes this association. Copyright © 2021. Published by Elsevier B.V.

Citation

Raffaella Belvedere, Emanuela Pessolano, Nunzia Novizio, Alessandra Tosco, Daniela Eletto, Amalia Porta, Amelia Filippelli, Francesco Petrella, Antonello Petrella. The promising pro-healing role of the association of mesoglycan and lactoferrin on skin lesions. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences. 2021 Aug 01;163:105886