Analysis of α-Synuclein in Exosomes.

Taiji Tsunemi, Yuta Ishiguro, Asako Yoroisaka, Nobutaka Hattori

Methods in molecular biology (Clifton, N.J.) 2021

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Alpha synuclein (α-Syn), a presynaptic protein with unknown function, is accumulated in Lewy bodies/neurites that are one of the hallmark pathologies of Parkinson's disease (PD). Missense or multiplication mutations in SNCA, which codes α-Syn, result in a genetic form of PD, further indicating the involvement of α-Syn in PD pathogenesis. Recent pathological and experimental studies suggest that α-Syn possesses a secretory feature, as it is detected in the culture media, in the cerebrospinal fluid, and even in the blood. Secreted α-Syn can spread throughout the body and invade the CNS, disseminating the α-Syn associated pathology. Exosomes are small extracellular vesicles that carry many proteins, lipids, or miRNA. We and others have discovered α-Syn in exosomes and revealed that exosomes may regulate intracellular α-Syn levels by transporting outside the cells. In this chapter, we describe a protocol to measure α-Syn levels in exosomes.

Citation

Taiji Tsunemi, Yuta Ishiguro, Asako Yoroisaka, Nobutaka Hattori. Analysis of α-Synuclein in Exosomes. Methods in molecular biology (Clifton, N.J.). 2021;2322:41-45