Correlation Engine 2.0
Clear Search sequence regions


  • and diseases (1)
  • bryopsida (1)
  • chloroplasts (2)
  • gene (1)
  • genomes (3)
  • human (1)
  • organelle (7)
  • physcomitrella (2)
  • plant (3)
  • plant proteins (2)
  • RECA (1)
  • RECG (1)
  • Sizes of these terms reflect their relevance to your search.

    Destabilization of organelle genomes causes organelle dysfunction that appears as abnormal growth in plants and diseases in human. In plants, loss of the bacterial-type homologous recombination repair (HRR) factors RECA and RECG induces organelle genome instability. In this study, we show the landscape of organelle genome instability in Physcomitrella patens HRR knockout mutants by deep sequencing in combination with informatics approaches. Genome-wide maps of rearrangement positions in the organelle genomes, which exhibited prominent mutant-specific patterns, were highly biased in terms of direction and location and often associated with dramatic variation in read depth. The rearrangements were location-dependent and mostly derived from the asymmetric products of microhomology-mediated recombination. Our results provide an overall picture of organelle-specific gross genomic rearrangements in the HRR mutants, and suggest that chloroplasts and mitochondria share common mechanisms for replication-related rearrangements.

    Citation

    Masaki Odahara, Kensuke Nakamura, Yasuhiko Sekine, Taku Oshima. Ultra-deep sequencing reveals dramatic alteration of organellar genomes in Physcomitrella patens due to biased asymmetric recombination. Communications biology. 2021 May 27;4(1):633

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 34045660

    View Full Text