Fluorescent Labeling of the Nuclear Envelope Without Relying on Inner Nuclear Membrane Proteins.

The nuclear envelope (NE), a double membrane that separates nuclear components from the cytoplasm, undergoes a breakdown and reformation during cell division. To trace NE dynamics, the NE needs to be labeled with a fluorescent marker, and for this purpose, markers based on inner nuclear membrane (INM) proteins are normally used. However, NE labeling with INM proteins has some limitations. Here, we introduce a protocol for fluorescent labeling and imaging of NE that does not rely on INM proteins, along with protocols for simultaneously imaging two nuclear components and for time-lapse imaging of labeled cells.

Citation

Toshiyuki Taniyama, Shinji Sueda. Fluorescent Labeling of the Nuclear Envelope Without Relying on Inner Nuclear Membrane Proteins. Methods in molecular biology (Clifton, N.J.). 2021;2274:3-14

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PMID: 34050457

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