Correlation Engine 2.0
Clear Search sequence regions

Sizes of these terms reflect their relevance to your search.

The nuclear envelope (NE), a double membrane that separates nuclear components from the cytoplasm, undergoes a breakdown and reformation during cell division. To trace NE dynamics, the NE needs to be labeled with a fluorescent marker, and for this purpose, markers based on inner nuclear membrane (INM) proteins are normally used. However, NE labeling with INM proteins has some limitations. Here, we introduce a protocol for fluorescent labeling and imaging of NE that does not rely on INM proteins, along with protocols for simultaneously imaging two nuclear components and for time-lapse imaging of labeled cells.


Toshiyuki Taniyama, Shinji Sueda. Fluorescent Labeling of the Nuclear Envelope Without Relying on Inner Nuclear Membrane Proteins. Methods in molecular biology (Clifton, N.J.). 2021;2274:3-14

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 34050457

View Full Text