L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma.

Maia Norman, Dmitry Ter-Ovanesyan, Wendy Trieu, Roey Lazarovits, Emma J K Kowal, Ju Hyun Lee, Alice S Chen-Plotkin, Aviv Regev, George M Church, David R Walt

Nature methods 2021 Jun

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L1CAM is a transmembrane protein expressed on neurons that was presumed to be found on neuron-derived extracellular vesicles (NDEVs) in human biofluids. We developed a panel of single-molecule array assays to evaluate the use of L1CAM for NDEV isolation. We demonstrate that L1CAM is not associated with extracellular vesicles in human plasma or cerebrospinal fluid and therefore recommend against its use as a marker in NDEV isolation protocols.

Citation

Maia Norman, Dmitry Ter-Ovanesyan, Wendy Trieu, Roey Lazarovits, Emma J K Kowal, Ju Hyun Lee, Alice S Chen-Plotkin, Aviv Regev, George M Church, David R Walt. L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma. Nature methods. 2021 Jun;18(6):631-634