Cytological and morphology characteristics of natural microsporogenesis within Camellia oleifera.

Camellia oleifera is believed to exhibit a complex intraspecific polyploidy phenomenon. Abnormal microsporogenesis can promote the formation of unreduced gametes in plants and lead to sexual polyploidy, so it is hypothesized that improper meiosis probably results in the formation of natural polyploidy in Camellia oleifera. In this study, based on the cytological observation of meiosis in pollen mother cells (PMCs), we found natural 2n pollen for the first time in Camellia oleifera, which may lead to the formation of natural polyploids by sexual polyploidization. Additionally, abnormal cytological behaviour during meiosis, including univalent chromosomes, extraequatorial chromosomes, early segregation, laggard chromosomes, chromosome stickiness, asynchronous meiosis and deviant cytokinesis (monad, dyads, triads), was observed, which could be the cause of 2n pollen formation. Moreover, we confirmed a relationship among the length-width ratio of flower buds, stylet length and microsporogenesis. This result suggested that we can immediately determine the microsporogenesis stages by phenotypic characteristics, which may be applicable to breeding advanced germplasm in Camellia oleifera. The online version contains supplementary material available at 10.1007/s12298-021-01002-5. © Prof. H.S. Srivastava Foundation for Science and Society 2021.

Citation

Xiaoyu Zhang, Hailang Tong, Zhiqiang Han, Long Huang, Jing Tian, Zhixing Fu, Yunyi Wu, Ting Wang, Deyi Yuan. Cytological and morphology characteristics of natural microsporogenesis within Camellia oleifera. Physiology and molecular biology of plants : an international journal of functional plant biology. 2021 May;27(5):959-968

PMID: 34092947

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