Detection of bladder cancer with aberrantly fucosylated ITGA3.

Md Khirul Islam, Parvez Syed, Bert Dhondt, Kamlesh Gidwani, Kim Pettersson, Urpo Lamminmäki, Janne Leivo

Analytical biochemistry 2021 Sep 01

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We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-doped-nanoparticles (Eu3+-NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCa-derived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay. Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.

Citation

Md Khirul Islam, Parvez Syed, Bert Dhondt, Kamlesh Gidwani, Kim Pettersson, Urpo Lamminmäki, Janne Leivo. Detection of bladder cancer with aberrantly fucosylated ITGA3. Analytical biochemistry. 2021 Sep 01;628:114283