Inhibition of Haemophilus parasuis by berberine and proteomic studies of its mechanism of action.

Yue Jia, Changqi Hao, Qin Yang, Wanjiang Zhang, Gang Li, Siguo Liu, Xin Hua

Research in veterinary science 2021 Sep

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Haemophilus parasuis is the main agent of Glässer's disease, which causes substantial losses in pig production. However, the pathogenic mechanism and virulence factors of H. parasuis have not been fully determined. In this study, berberine is shown to have a good therapeutic effect in vivo against H. parasuis; the minimal inhibitory concentration (MIC) in vitro was 2 μg/mL. Berberine inhibited H. parasuis adhesion to and invasion of PK-15 pig kidney cells. Proteomics studies of H. parasuis after berberine treatment identified a total of 97 differentially-expressed proteins; 35 upregulated and 62 downregulated. Bioinformatics analysis showed that berberine may inhibit the growth of H. parasuis by affecting outer membrane proteins, transferrins, and energy metabolism. This study provides a basis for the development of new antibacterial agents. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Yue Jia, Changqi Hao, Qin Yang, Wanjiang Zhang, Gang Li, Siguo Liu, Xin Hua. Inhibition of Haemophilus parasuis by berberine and proteomic studies of its mechanism of action. Research in veterinary science. 2021 Sep;138:62-68