High-resolution analysis of chromosome conformation in hyperthermophilic archaea.

Naomichi Takemata, Stephen D Bell

STAR protocols 2021 Jun 18

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Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep sequencing for the hyperthermophilic archaeal genus Sulfolobus. Instead of using restriction enzymes compatible with fill-in labeling, this protocol uses the 4-bp blunt cutter AluI to generate high-resolution (up to 2 kb) contact maps from Sulfolobus species. For complete details on the use and execution of this protocol, please refer to Takemata and Bell (2021). © 2021 The Author(s).

Citation

Naomichi Takemata, Stephen D Bell. High-resolution analysis of chromosome conformation in hyperthermophilic archaea. STAR protocols. 2021 Jun 18;2(2):100562