Efficacy of Silene arenosa extract on acetylcholinesterase in Bungarus sindanus (krait) venom.

To examine the efficacy of Silene arenosa extract on acetylcholinesterase (AChE) of krait (Bungarus Sindanus) snake venom. The present project designed to evaluate the inhibition of AChE by following standard procedures. Statistical analysis of the results showed that Silene arenosa exerted 73% inhibition against the krait venom acetylcholinesterase at fixed substrate acetylcholine (ACh) concentration (0.5 mM). Kinetic analysis using the Lineweaver Burk plot revealed that Silene arenosa caused a competitive type of inhibition i.e. Km values increased from 26.6 to 93.3 mM (26.6% to 93.3%) and Vmax remained constant in a concentration-dependent manner. Silene arenosa competes with the substrate to bind at the active site of the enzyme. The Kmapp of venom AChE for Silene arenosa increased from 60% to 81.6% and the Vmaxapp remains constant. Ki (inhibition constant was estimated to be 48 μg for snake venom; while the Km (Michaelis-Menten constant of AChE- substrate into AChE and product) was estimated to be 0.5 mM. The IC50 of AchE calculated for Silene arenosa was 67 μg. The present results suggest that Silene arenosa extract can be considered as an inhibitor of snake venom AChE.

Citation

Adil Shah, Mushtaq Ahmed, Naila Sher, Nadia Mushtaq, Rahmat-Ali Khan, Fozia, Midrarullah. Efficacy of Silene arenosa extract on acetylcholinesterase in Bungarus sindanus (krait) venom. Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan. 2021 Jun;41(3):349-354

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PMID: 34114390

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