Detection of Posttranslational Modification Autoantibodies Using Peptide Microarray.

Meng Li, Hongye Wang, Jiayu Dai, Meng Xu, Jianhua Liu, Jing Ren, Xiaosong Qin, Xianjiang Kang, Xiaobo Yu

Methods in molecular biology (Clifton, N.J.) 2021

filter terms:

Autoantibodies are humoral antibodies against self-proteins and play vital roles in maintaining the homeostasis. Autoantibodies can also target posttranslational modifications (PTMs) of proteins and the identification of new PTM autoantibodies is important to identify biomarkers for the early diagnosis of cancer and autoimmune diseases. In this chapter, we describe a method to detect PTM autoantibodies using citrullinated peptide microarray as an example. This method can be used to screen serum autoantibodies for different human diseases.

Citation

Meng Li, Hongye Wang, Jiayu Dai, Meng Xu, Jianhua Liu, Jing Ren, Xiaosong Qin, Xianjiang Kang, Xiaobo Yu. Detection of Posttranslational Modification Autoantibodies Using Peptide Microarray. Methods in molecular biology (Clifton, N.J.). 2021;2344:99-106