Reliability and validity of a standardised ultrasound examination protocol to quantify vastus lateralis muscle.

To evaluate the reliability and validity of a standardized ultrasound examination protocol for measuring vastus lateralis muscle size. Prospective cohort study. Sixteen staff members of the university hospital of Heidelberg. Muscle thickness, cross-sectional area and subcutaneous adipose tissue thickness were measured at 3 standardized sites on the right and left vastus lateralis muscle. Ultrasound measurements were collected by 2 independent investigators on 2 different days and compared with magnetic resonance imaging measurements. Intraclass correlation coefficients (ICC) for intra- and inter-rater reliability showed very good closeness of agreement for all parameters (ICC = 0.929-0.994, p < 0.001). Muscle thickness and subcutaneous adipose tissue thickness ultrasound and magnetic resonance imaging measurements revealed good to very good closeness of agreement (ICC = 0.835-0.969, p < 0.001), whereas cross-sectional area showed only average closeness of agreement (ICC = 0.727, p < 0.001). A strong predictive positive correlation for ultrasound and magnetic resonance imaging-based measurements of cross-sectional area was found (R² = 0.793, p < 0.001). By standardization of an examination protocol, quantitative vastus lateralis muscle ultrasound proved to be a reliable method for assessing vastus lateralis muscle size. Furthermore, this protocol is valid for measuring muscle thickness and subcutaneous adipose tissue thickness, although there seems to be a systematic underestimation of cross-sectional area depending on subcutaneous adipose tissue thickness.

Citation

Theresa Maria Betz, Michaela Wehrstein, Fabian Preisner, Martin Bendszus, Birgit Friedmann-Bette. Reliability and validity of a standardised ultrasound examination protocol to quantify vastus lateralis muscle. Journal of rehabilitation medicine. 2021 Jul 06;53(7):jrm00212

Mesh Tags

PMID: 34121129

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