Dual-Targeting Nanoprobe for Early Diagnosis of Pheochromocytoma Though Coinstantaneous Identification of Circulating Tumor Cells.

Xiaoya Liu, Xicheng Zhang, Junyan Tang, Yang Meng, Liping Zhao, Wenjing Shi, Feifei Tao, Zigui Kan, Fei Wang, Caolong Li

Analytical chemistry 2021 Jul 06

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Circulating tumor cells (CTCs) play a pivotal role in the early diagnosis of pheochromocytoma (PCC). Herein, we fabricated a new dual-targeting nanoprobe for coinstantaneous identification of rare PCC-CTCs from peripheral blood via targeting the norepinephrine transporter (NET) and somatostatin receptor SSTR2 overexpressed on the surface of PCC cells. Meta-iodobenzylguanidine (MIBG) functionalized magnetic Fe3O4 and octreotide (DOTA) decorated signal amplification Ag@SiO2 nanosphere were used to capture and detect PCC-CTCs by binding to NET and SSTR2. The proposed dual-targeting sensor achieved good reproducibility and high sensitivity for the monitoring of PC12 in the concentration range from 5 to 5 × 104 cells mL-1, with detection limits of 2 cell/mL. This strategy opens a new approach for simple, sensitive, and rapid determination of PCC biomarkers, which shows great potential in early diagnosis, prognosis, and therapeutic evaluation of PCC.

Citation

Xiaoya Liu, Xicheng Zhang, Junyan Tang, Yang Meng, Liping Zhao, Wenjing Shi, Feifei Tao, Zigui Kan, Fei Wang, Caolong Li. Dual-Targeting Nanoprobe for Early Diagnosis of Pheochromocytoma Though Coinstantaneous Identification of Circulating Tumor Cells. Analytical chemistry. 2021 Jul 06;93(26):9036-9040