Immunization with Pneumocystis carinii A121-85 antigen activates immune function against P. carinii.

Tong Tong, Zhongxin Wang, Yuanhong Xu, Jilu Shen

BMC immunology 2021 Jun 27

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Pneumocystis pneumonia (PcP), which is caused by Pneumocystis carinii, is a life-threatening infection that affects immunocompromised individuals. Unfortunately, chemoprophylaxis and dapsone are only effective for half of the patients with PcP, indicating that additional preventive methods are needed. We predicated the pneumocystis surface protein A12 sequence 1-85 by DNAStar software and BepiPred, and identified it as a potential vaccine candidate by bioresearch. We used recombinant A121-85 as antigen to immunized mice and detected serum titer of IgG, expression of inflammatory factors by EILSA, qRT-PCR and flow cytometry. Our results showed that immunization with recombinant A121-85 increased the serum titer of IgG, promoted the secretion of T lymphocytes, increased the expression of inflammatory factors, and elevated lung inflammatory injury in mice. Our findings suggest that A121-85 is a potential vaccine target for preventing Pneumocystis carinii. The evaluation of A121-85-elicited antibodies in the prevention of PcP in humans deserves further investigation.

Citation

Tong Tong, Zhongxin Wang, Yuanhong Xu, Jilu Shen. Immunization with Pneumocystis carinii A121-85 antigen activates immune function against P. carinii. BMC immunology. 2021 Jun 27;22(1):40