Correlation Engine 2.0
Clear Search sequence regions


  • disease mouth (2)
  • dna vaccines (1)
  • elements (1)
  • foot (5)
  • foreign (1)
  • gene (3)
  • gene viral (1)
  • human (2)
  • models molecular (1)
  • mouth (5)
  • mrna (3)
  • picornaviruses (3)
  • plasmids (3)
  • polio (1)
  • research (1)
  • rna (4)
  • rna ii (1)
  • rna polymerase (1)
  • rna viruses (1)
  • vaccines (1)
  • Sizes of these terms reflect their relevance to your search.

    Picornaviruses are non-enveloped, single-stranded RNA viruses that cause highly contagious diseases, such as polio and hand, foot-and-mouth disease (HFMD) in human, and foot-and-mouth disease (FMD) in animals. Reverse genetics and minigenome of picornaviruses mainly depend on in vitro transcription and RNA transfection; however, this approach is inefficient due to the rapid degradation of RNA template. Although DNA-based reverse genetics systems driven by mammalian RNA polymerase I and/or II promoters display the advantage of rescuing the engineered FMDV, the enzymatic functions are restricted in the nuclear compartment. To overcome these limitations, we successfully established a novel DNA-based vector, namely pKLS3, an FMDV minigenome containing the minimum cis-acting elements of FMDV essential for intracytoplasmic transcription and translation of a foreign gene. A combination of pKLS3 minigenome and the helper plasmids yielded the efficient production of uncapped-green florescent protein (GFP) mRNA visualized in the transfected cells. We have demonstrated the application of the pKLS3 for cell-based antiviral drug screening. Not only is the DNA-based FMDV minigenome system useful for the FMDV research and development but it could be implemented for generating other picornavirus minigenomes. Additionally, the prospective applications of this viral minigenome system as a vector for DNA and mRNA vaccines are also discussed.

    Citation

    Ploypailin Semkum, Challika Kaewborisuth, Nattarat Thangthamniyom, Sirin Theerawatanasirikul, Chalermpol Lekcharoensuk, Payuda Hansoongnern, Pongrama Ramasoota, Porntippa Lekcharoensuk. A Novel Plasmid DNA-Based Foot and Mouth Disease Virus Minigenome for Intracytoplasmic mRNA Production. Viruses. 2021 Jun 01;13(6)

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 34205958

    View Full Text