Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq.

Martin Philpott, Jonathan Watson, Anjan Thakurta, Tom Brown, Tom Brown, Udo Oppermann, Adam P Cribbs

Nature biotechnology 2021 Dec

filter terms:

Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized using dimeric nucleotide building blocks that allow error detection. We illustrate the use of the method for evaluating barcode assignment accuracy, differential isoform usage in myeloma cell lines, and fusion transcript detection in a sarcoma cell line. © 2021. The Author(s).

Citation

Martin Philpott, Jonathan Watson, Anjan Thakurta, Tom Brown, Tom Brown, Udo Oppermann, Adam P Cribbs. Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq. Nature biotechnology. 2021 Dec;39(12):1517-1520