U1 small nuclear ribonucleoprotein is essential for early larval development in silkworm, Bombyx mori.

Xiaoqian Zhang, Dehong Yang, Xu Yang, Xinran Zhang, Yong Zhang, Yongping Huang, Shengxiang Zhang, Zulian Liu

Insect science 2022 Apr

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U1 small nuclear ribonucleoproteins (U1 snRNP) associates with 5' splice sites in the form of ribonucleoprotein particles and is highly conserved among species. The physiological functions of U1 snRNP in a lepidopteran model insect Bombyx mori is mostly unknown. Here, we showed that U1 snRNP plays an important role in the development of silkworm. Knockout of U1 snRNP in silkworm showed either delayed or stationary 1st instar larva development compared with the wild-type group. U1 snRNP deletion mutants exhibited abnormal cellular phenotypes with enlarged cell nucleus, scant cytoplasm and enlarged nuclei. RNA-seq analysis revealed that genes involved in metabolic pathway, biosynthesis of secondary metabolites and steroid hormone biosynthesis were significantly affected by U1 snRNP depletion. Taken together, our study suggests that U1 snRNP homeostasis plays an important role in silkworm development. © 2021 Institute of Zoology, Chinese Academy of Sciences.

Citation

Xiaoqian Zhang, Dehong Yang, Xu Yang, Xinran Zhang, Yong Zhang, Yongping Huang, Shengxiang Zhang, Zulian Liu. U1 small nuclear ribonucleoprotein is essential for early larval development in silkworm, Bombyx mori. Insect science. 2022 Apr;29(2):379-387