G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p.

Jun Gao, Zhaofeng Gao, Andrea A Putnam, Alicia K Byrd, Sarah L Venus, John C Marecki, Andrea D Edwards, Haley M Lowe, Eckhard Jankowsky, Kevin D Raney

Chemical communications (Cambridge, England) 2021 Jul 27

filter terms:

G-quadruplex DNA interacts with the N-terminal intrinsically disordered domain of the DEAD-box helicase Ded1p, diminishing RNA unwinding activity but enhancing liquid-liquid phase separation of Ded1p in vitro and in cells. The data highlight multifaceted effects of quadruplex DNA on an enzyme with intrinsically disordered domains.

Citation

Jun Gao, Zhaofeng Gao, Andrea A Putnam, Alicia K Byrd, Sarah L Venus, John C Marecki, Andrea D Edwards, Haley M Lowe, Eckhard Jankowsky, Kevin D Raney. G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p. Chemical communications (Cambridge, England). 2021 Jul 27;57(60):7445-7448