A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK-2 cells via regulating NADPH/oxidase-TGF-β/Smad signaling pathway.

Qinwen Wang, Xinxin Dai, Xiang Xiang, Zhuo Xu, Shulan Su, Dandan Wei, Tianyao Zheng, Er-Xin Shang, Dawei Qian, Jin-Ao Duan

Phytotherapy research : PTR 2021 Sep

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This study was designed to investigate the protective effects and mechanisms of acteoside on DKD in diabetes male db/db mice and high glucose-induced HK-2 cells. The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and acteoside group. We observed the natural product of acteoside exhibiting a significant effect in renal protection through analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK-2 cells subjected to high glucose were used in invitro experiments. The molecular mechanisms of them were investigated by RT-PCR and western blot. Acteoside prevents high glucose-induced HK-2 cells and diabetes db/db mice by inhibiting NADPH/oxidase-TGF-β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We discovered the natural product of acteoside exhibiting a significant effect in renal protection. This study paved the way for further exploration of pathogenesis, early diagnosis, and development of a new therapeutic agent for DKD. © 2021 John Wiley & Sons Ltd.

Citation

Qinwen Wang, Xinxin Dai, Xiang Xiang, Zhuo Xu, Shulan Su, Dandan Wei, Tianyao Zheng, Er-Xin Shang, Dawei Qian, Jin-Ao Duan. A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK-2 cells via regulating NADPH/oxidase-TGF-β/Smad signaling pathway. Phytotherapy research : PTR. 2021 Sep;35(9):5227-5240