Zhuojia Xu, Yaqi Deng, Zhumin Zhang, Wenjing Ma, Wanjin Li, Liuqing Wen, Tiehai Li
The Journal of organic chemistry 2021 Aug 06A diversity-oriented chemoenzymatic approach for the collective preparation of sulfated core 2 O-GalNAc glycans and their nonsulfated counterparts was described. A sulfated trisaccharide and a nonsulfated trisaccharide were chemically synthesized by combining flexible protected group manipulations and sequential one-pot glycosylations. The divergent enzymatic extension of these two trisaccharides, using a panel of robust glycosyltransferases that can recognize sulfated substrates and differentiating the branches with specifically designed glycosylation sequences to achieve regioselective sialylation, provided 36 structurally well-defined O-GalNAc glycans.
Zhuojia Xu, Yaqi Deng, Zhumin Zhang, Wenjing Ma, Wanjin Li, Liuqing Wen, Tiehai Li. Diversity-Oriented Chemoenzymatic Synthesis of Sulfated and Nonsulfated Core 2 O-GalNAc Glycans. The Journal of organic chemistry. 2021 Aug 06;86(15):10819-10828
PMID: 34254798
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