Sufu negatively regulates both initiations of centrosome duplication and DNA replication.

Tenghan Zhuang, Boyan Zhang, Yihong Song, Fan Huang, Wangfei Chi, Guangwei Xin, Ziyu Zhang, Steven Y Cheng, Qing Jiang, Chuanmao Zhang

Proceedings of the National Academy of Sciences of the United States of America 2021 Jul 13

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Centrosome duplication and DNA replication are two pivotal events that higher eukaryotic cells use to initiate proliferation. While DNA replication is initiated through origin licensing, centrosome duplication starts with cartwheel assembly and is partly controlled by CP110. However, the upstream coordinator for both events has been, until now, a mystery. Here, we report that suppressor of fused protein (Sufu), a negative regulator of the Hedgehog (Hh) pathway playing a significant role in restricting the trafficking and function of glioma-related (Gli) proteins, acts as an upstream switch by facilitating CP110 phosphorylation by CDK2, promoting intranuclear Cdt1 degradation and excluding prereplication complex (pre-RC) components from chromosomes, independent of its canonical function in the Hh pathway. We found that Sufu localizes to both the centrosome and the nucleus and that knockout of Sufu induces abnormalities including centrosome amplification, increased nuclear size, multipolar spindle formation, and polyploidy. Serum stimulation promotes the elimination of Sufu from the centrosome by vesicle release at the ciliary tip and from the nucleus via protein degradation, which allows centrosome duplication and DNA replication to proceed. Collectively, this work reveals a mechanism through which Sufu negatively regulates the G1-S transition.

Citation

Tenghan Zhuang, Boyan Zhang, Yihong Song, Fan Huang, Wangfei Chi, Guangwei Xin, Ziyu Zhang, Steven Y Cheng, Qing Jiang, Chuanmao Zhang. Sufu negatively regulates both initiations of centrosome duplication and DNA replication. Proceedings of the National Academy of Sciences of the United States of America. 2021 Jul 13;118(28)