Biomarkers of geno- and cytotoxicity in the native broad-snouted caiman (Caiman latirostris): Chromosomal aberrations and mitotic index.

Camila Felisa Chacón, Evelyn Cecilia López González, Gisela Laura Poletta

Mutation research. Genetic toxicology and environmental mutagenesis 2021 Jul

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We evaluated the sensitivity of the chromosomal aberration (CA) and mitotic index (MI) assays on peripheral blood lymphocytes (PBLs) of Caiman latirostris, following ex vivo exposure to the alkylating agent, MMS. Two concentrations of MMS were tested in cultured peripheral blood. Relative to controls, MMS exposure reduced the number of metaphases observed, but both the numbers of cells with MN and the percentages of aberrant metaphases increased. The types of CA identified were chromosome and chromatid breaks, chromosomal rearrangements, monosomies, and nullisomies, with significantly higher values in the MMS-exposed groups. The incorporation of the MI and CA tests in C. latirostris can provide information on damage caused by xenobiotic exposures. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Camila Felisa Chacón, Evelyn Cecilia López González, Gisela Laura Poletta. Biomarkers of geno- and cytotoxicity in the native broad-snouted caiman (Caiman latirostris): Chromosomal aberrations and mitotic index. Mutation research. Genetic toxicology and environmental mutagenesis. 2021 Jul;867:503353