Comparison of techniques for the extraction of Camptothecin from Tabernaemontana species.

Pallavi Karanje, Vishwajeet Ghorpade, Ajay Namdeo, Rajendra Doijad, Abhijit Karanje

Natural product research 2023 Jan

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Extraction methods like maceration, ultrasonication, vortex mixer, soxhlet extraction and microwave assisted extraction (MAE), were evaluated for the extraction of Camptothecin (CPT) from the leaves and stem of Tabernaemontana alternifolia, Tabernaemontana divaricata and Tabernaemontana citrifolia. The extracts were analyzed by high performance thin layer chromatography (HPTLC). The results show that the leaves of Tabernaemontana alternifolia exhibited highest yield of CPT as compared to the other species. MAE was the most efficient extraction method with CPT extraction yield of 0.154 ± 0.004% w/w from Tabernaemontana alternifolia leaves followed by Soxhlet extraction, sonication, maceration, and vortex extraction methods.

Citation

Pallavi Karanje, Vishwajeet Ghorpade, Ajay Namdeo, Rajendra Doijad, Abhijit Karanje. Comparison of techniques for the extraction of Camptothecin from Tabernaemontana species. Natural product research. 2023 Jan;37(2):322-327