Mitochondrial DNA deletion-dependent podocyte injuries in Mito-miceΔ, a murine model of mitochondrial disease.

Shuzo Kaneko, Joichi Usui, Masahiro Hagiwara, Tatsuya Shimizu, Ryota Ishii, Mayumi Takahashi-Kobayashi, Mikiko Kageyama, Kazuto Nakada, Jun-Ichi Hayashi, Kunihiro Yamagata

Experimental animals 2022 Feb 09

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Focal segmental glomerulosclerosis (FSGS) is a major renal complication of human mitochondrial disease. However, its pathogenesis has not been fully explained. In this study, we focused on the glomerular injury of mito-miceΔ and investigated the pathogenesis of their renal involvement. We analyzed biochemical data and histology in mito-miceΔ. The proteinuria began to show in some mito-miceΔ with around 80% of mitochondrial DNA deletion, then proteinuria developed dependent with higher mitochondrial DNA deletion, more than 90% deletion. Mito-miceΔ with proteinuria histologically revealed FSGS. Immunohistochemistry demonstrated extensive distal tubular casts due to abundant glomerular proteinuria. Additionally, the loss of podocyte-related protein and podocyte's number were found. Therefore, the podocyte injuries and its depletion had a temporal relationship with the development of proteinuria. This study suggested mitochondrial DNA deletion-dependent podocyte injuries as the pathogenesis of renal involvement in mito-miceΔ. The podocytes are the main target of mitochondrial dysfunction originated from the accumulation of mitochondrial DNA abnormality in the kidney.

Citation

Shuzo Kaneko, Joichi Usui, Masahiro Hagiwara, Tatsuya Shimizu, Ryota Ishii, Mayumi Takahashi-Kobayashi, Mikiko Kageyama, Kazuto Nakada, Jun-Ichi Hayashi, Kunihiro Yamagata. Mitochondrial DNA deletion-dependent podocyte injuries in Mito-miceΔ, a murine model of mitochondrial disease. Experimental animals. 2022 Feb 09;71(1):14-21