Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma].

Shuiqin Fang, Cheng Liu, Junfei Ma, Shuaishuai Yan, Dongpo Xu, Qing Liu

Sheng wu gong cheng xue bao = Chinese journal of biotechnology 2021 Jul 25

filter terms:

Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.

Citation

Shuiqin Fang, Cheng Liu, Junfei Ma, Shuaishuai Yan, Dongpo Xu, Qing Liu. Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology. 2021 Jul 25;37(7):2293-2306