Phytochemical screening, green synthesis of gold nanoparticles, and antibacterial activity using seeds extract of Ricinus communis L.

Taj Ur Rahman, Hammad Khan, Wajiha Liaqat, Muhammad Aurang Zeb

Microscopy research and technique 2022 Jan

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In the current research study, the phytochemical tests of the Ricinus communis L methanolic extract detected the presence of coumarin, reducing sugar, emodines, terpenoids, flavonoids, and steroids while water extract showed the presence of alkaloids, terpenoids, steroids, and soluble starch. The methanolic extract was further subjected to synthesize Au nanoparticles. The synthesized nanoparticles were confirmed by UV-Vis through the appearance of a peak at 550 nm which correspond to the existence of Au nanoparticles. The size and morphology of synthesized nanoparticle were further studied by the dynamic light scattering technique which shows that nanoparticles were in the range of 100 nm which were further confirmed by transmission electron microscopy. The synthesized nanoparticles and extracts were tested against different bacterial strains. The results revealed that methanolic extract showed maximum inhibition of 19.5 mm against Klebsiella pneumoniae and 20.33 mm against Bacillus cereus while water extract showed maximum inhibition of 18.16 mm against Pseudomonas aeruginosa and 19.33 mm against B. cereus. On the other hand, Au nanoparticles showed maximum inhibition of 18.5 mm against Escherichia coli. © 2021 Wiley Periodicals LLC.

Citation

Taj Ur Rahman, Hammad Khan, Wajiha Liaqat, Muhammad Aurang Zeb. Phytochemical screening, green synthesis of gold nanoparticles, and antibacterial activity using seeds extract of Ricinus communis L. Microscopy research and technique. 2022 Jan;85(1):202-208