Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture.

Andrea Vega, Isabel Fredes, José O'Brien, Zhouxin Shen, Krisztina Ötvös, Rashed Abualia, Eva Benkova, Steven P Briggs, Rodrigo A Gutiérrez

EMBO reports 2021 Sep 06

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Nitrate commands genome-wide gene expression changes that impact metabolism, physiology, plant growth, and development. In an effort to identify new components involved in nitrate responses in plants, we analyze the Arabidopsis thaliana root phosphoproteome in response to nitrate treatments via liquid chromatography coupled to tandem mass spectrometry. 176 phosphoproteins show significant changes at 5 or 20 min after nitrate treatments. Proteins identified by 5 min include signaling components such as kinases or transcription factors. In contrast, by 20 min, proteins identified were associated with transporter activity or hormone metabolism functions, among others. The phosphorylation profile of NITRATE TRANSPORTER 1.1 (NRT1.1) mutant plants was significantly altered as compared to wild-type plants, confirming its key role in nitrate signaling pathways that involves phosphorylation changes. Integrative bioinformatics analysis highlights auxin transport as an important mechanism modulated by nitrate signaling at the post-translational level. We validated a new phosphorylation site in PIN2 and provide evidence that it functions in primary and lateral root growth responses to nitrate. © 2020 The Authors. Published under the terms of the CC BY NC ND 4.0 license.

Citation

Andrea Vega, Isabel Fredes, José O'Brien, Zhouxin Shen, Krisztina Ötvös, Rashed Abualia, Eva Benkova, Steven P Briggs, Rodrigo A Gutiérrez. Nitrate triggered phosphoproteome changes and a PIN2 phosphosite modulating root system architecture. EMBO reports. 2021 Sep 06;22(9):e51813