Controlled density glycodendron microarrays for studying carbohydrate-lectin interactions.

Antonio Di Maio, Anna Cioce, Silvia Achilli, Michel Thépaut, Corinne Vivès, Franck Fieschi, Javier Rojo, Niels-C Reichardt

Organic & biomolecular chemistry 2021 Sep 14

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Glycodendron microarrays with defined valency have been constructed by on-chip synthesis on hydrophobic indium tin oxide (ITO) coated glass slides and employed in lectin-carbohydrate binding studies with several plant and human lectins. Glycodendrons presenting sugar epitopes at different valencies were prepared by spotwise strain-promoted azide-alkyne cycloaddition (SPAAC) between immobilised cyclooctyne dendrons and azide functionalised glycans. The non-covalent immobilisation of dendrons on the ITO surface by hydrophobic interaction allowed us to study dendron surface density and SPAAC conversion rate by in situ MALDI-TOF MS analysis. By diluting the dendron surface density we could study how the carbohydrate-lectin interactions became exclusively dependant on the valency of the immobilised glycodendron.

Citation

Antonio Di Maio, Anna Cioce, Silvia Achilli, Michel Thépaut, Corinne Vivès, Franck Fieschi, Javier Rojo, Niels-C Reichardt. Controlled density glycodendron microarrays for studying carbohydrate-lectin interactions. Organic & biomolecular chemistry. 2021 Sep 14;19(34):7357-7362