Echinococcus multilocularis infection induces UBE2N suppression via exosomal emu-miR-4989.

Mengting Cai, Juntao Ding, Yating Li, Guitian He, Jing Yang, Tingli Liu, Xiaola Guo, Xing Yang, Xiaoqiang Wang, William C Cho, Majid Fasihi Harandi, Yadong Zheng

Acta tropica 2021 Nov

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Echinococcus multilocularis metacestodes mainly reside in liver in humans and animals, and cause serious damages. UBE2N was herein shown to be downregulated in response to the infection. UBE2N was further shown to be predominantly expressed in the hepatocytes, which was also significantly downregulated during the infection. UBE2N was a target of emu-miR-4989, which was loaded into the exosomes secreted by parasites. These emu-miR-4989-encapsulating exosomes were internalized by hepatocytes, and induced a significant decrease of relative luciferase activity in the cells transfected with the construct containing a wild type of UBE2N 3'-UTR compared to the control (p < 0.05). These results demonstrate that emu-miR-4989 is involved in the UBE2N inhibition in the hepatocytes during E. multilocularis through exosomes. Copyright © 2021. Published by Elsevier B.V.

Citation

Mengting Cai, Juntao Ding, Yating Li, Guitian He, Jing Yang, Tingli Liu, Xiaola Guo, Xing Yang, Xiaoqiang Wang, William C Cho, Majid Fasihi Harandi, Yadong Zheng. Echinococcus multilocularis infection induces UBE2N suppression via exosomal emu-miR-4989. Acta tropica. 2021 Nov;223:106087