Facile synthesis of recyclable laccase-mineral hybrid complexes with enhanced activity and stability for biodegradation of Evans Blue dye.

Miaorong Zhang, Yan Zhang, Chuankai Yang, Chunyun Ma, Yuhang Zhang, Jianguo Tang

International journal of biological macromolecules 2021 Oct 01

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Two morphologies of laccase-mineral hybrid complexes, i.e., laccase-mineral hybrid nanoflowers (La-HNF) and nanopetals (La-HNP), were synthesized via biomineralization using Cu3 (PO4)2·3H2O as the mineral for Evans Blue (EB) dye biodegradation. XRD patterns and FT-IR spectra results revealed the successful immobilization of laccase via in-situ formed Cu3(PO4)2·3H2O crystals. Compared with free laccase, laccase-mineral hybrid complexes showed higher enzymatic activity due to the activation effect induced by copper ions of Cu3(PO4)2·3H2O, further, the improved kinetic parameters of laccase-mineral hybrid complexes could be ascribed to nanoscale-dispersed laccase molecules within hybrid complexes. For EB dye biodegradation, the reason why the biodegradation efficiency (94.9%) of La-HNF was higher than that (86.8%) of La-HNP could be synergistic effect of immobilized laccase within 3D hierarchical structure of La-HNF. In addition, the optimized biodegradation conditions (pH 4.6 and 40 °C) of La-HNF were obtained, moreover, 93.2% and 48.1% of EB dye were biodegraded by La-HNF after stored for 30 days and reused for 10 cycles, respectively, demonstrating La-HNF have good practicability. Copyright © 2021 Elsevier B.V. All rights reserved.

Citation

Miaorong Zhang, Yan Zhang, Chuankai Yang, Chunyun Ma, Yuhang Zhang, Jianguo Tang. Facile synthesis of recyclable laccase-mineral hybrid complexes with enhanced activity and stability for biodegradation of Evans Blue dye. International journal of biological macromolecules. 2021 Oct 01;188:783-789